Guanidine thiocyanate, BioReagent, for molecular biology

Code: 50981-100G D2-231

Application

Chaotropic agent and strong denaturant; solubilizes cells.

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It ...


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€178.00 100G
€218.94 inc. VAT
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Application

Chaotropic agent and strong denaturant; solubilizes cells.

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.

Packaging

100, 500 g in glass bottle

Principle

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

anion traceschloride (Cl-): ≤5000 mg/kg
assay≥99%
biological sourcesynthetic
cation tracesBi: ≤5 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, K: ≤50 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, Na: ≤5000 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Ca: ≤10 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
colorcolorless to white,
formpowder
gradefor molecular biology
impuritiesphosphatases, none detected, RNases, none detected, proteases, none detected, DNases, none detected
InChI keyZJYYHGLJYGJLLN-UHFFFAOYSA-N
InChI1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
mp120-122 °C (lit.), 115-122 °C
product lineBioReagent
Quality Level200
SMILES stringSC#N.NC(N)=N
solubilityH2O: 4 M at 20 °C, clear, colorless
storage temp.room temp
technique(s)RNA extraction: suitable
Cas Number593-84-0
Hazard Class8
Un Number1759
Pack GroupIII
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